Engineering works affecting Sheffield Supertram services will take place all day from Friday 20 April until Monday 23 April.
Replacement buses will be provided between Fitzalan Square and Meadowhall tram stops to minimise travel disruption for passengers.
Nunnery Square, Woodburn Road, Attercliffe, Arena, Valley Centertainment, Carbrook, Meadowhall South/Tinsley and Meadowhall Interchange stops will be closed on these days.
Yellow Route services will operate between Middlewood and Cricket Inn Road only. Purple Route services on Sunday 22 April will operate between Herdings Park and Cathedral only.
The engineering works, to replace the rails embedded in the highway, will take place on Shirland Lane crossing, next to Attercliffe tram stop.
Replacement bus service Y1 will run from Fitzalan Square via Cricket Inn Road, Woodburn Road, Staniforth Road and then via Attercliffe Road (A6178) towards Meadowhall, stopping at all bus stops along the route.
Passengers with valid tram tickets will be able to use the replacement buses at no extra cost.
Services will resume as normal on Tuesday 24 April.
Travel South Yorkshire is advising passengers to plan ahead and check before they travel at www.travelsouthyorkshire.com/journeyplanning or to follow @TravelSYorks, @TSYAlerts or @SCSupertram on Twitter for up-to-the-minute travel news.